Larva migrans cutánea a propósito de un caso / Cutaneous larva migrans taking into consideration a case

Introducción: la Larva migrans cutánea (LMC) es una enfermedad muy común en regiones tropicales, por lo que Cuba no está exenta de este tipo de infestación. Objetivo: relatar un caso de Larva migrans cutánea en un recién nacido de 18 días, visto durante estancia en Mozambique. Presentación del Caso: se presenta el caso de un recién nacido de 18 días, quien ingresa en el Servicio Infeccioso del Hospital Central de Beira, Provincia de Mozambique, con el posible diagnóstico de Meningoencefalitis y al que se le encuentra al examen físico lesiones eritematosas de trayecto lineal tortuosas a nivel de la región pre auricular, tórax y región glútea derecha, después de descartar otras entidades nosológicas. Conclusiones: se trata de una Larva migrans cutánea para lo que se impone tratamiento con cloruro de etilo y tiabendazol local. Presentó una evolución satisfactoria.

Introduction: cutaneous larva migrans is a very common disease in tropical regions that's why Cuba is included in the coontries in which this illness is present. Objective: talk about a cutaneous larva migrans case in an 18 days newborn, seen during the stay in Mozambique. Case Presentation: there is the case of a new born of 18 days who is addmited in the contagioos or infective Service of Beira Central Hospital, Mozambike province, with the possible diagnosis of the Meningoencephalitis, and within physical exam, physical lessions are found of the rind of erythematous of a acute lineal way at the level of the pre auricular region, thorax and de right buttock region. After discoonting others nosological entities. Conclussions: we are in the pressence of cutaneous Larva migrans and the local tiabendazol with cloruro of etilo tretment is impossed finding a notorioos satisfactory evolution after this.

CLSI broth microdilution method for testing susceptibility of Malasseziapachydermatis to thiabendazole / CLSI método de Microdiluição em Caldo para teste de suscetibilidade da Malassezia pachydermatis frente ao tiabendazol

Thiabendazole, classified as antiparasitic and also used as an antifungal drug, can be found as otological solution indicated for treatment of parasitic and fungal external otitis in small animals. Malassezia pachydermatis is a yeast recognized as a normal inhabitant on the skin and mucous membranes of dogs andcats. However, it is considered an opportunistic agent that causes external otitis and dermatitis in these animals. The aim of this study was to evaluate the in vitro effect of thiabendazole against 51 isolates of M. pachydermatis using the CLSI Broth Microdilution method that has been adapted for this yeast species (NCCLS, 2002). Based on this test, the Minimum Inhibitory Concentrations (MIC) of thiabendazol was calculated. Subsequently, the susceptibility of each isolate against this antifungal was determined. It wasobserved that the MIC of thiabendazole against M. pachydermatis ranged from 0.03 to > 4 μg/mL. A total of 13.7% of the isolates were found to be resistant, 47.1% were intermediate and 39.2% were sensitive to the drug. The rate of resistance of the yeasts against thiabendazole was similar to the results previously obtained with other antifungals, while the adapted broth microdilution technique used in this study proved to be efficient.

Tiabendazol, um fármaco classificado como antiparasitário e também usado como antifúngico, pode ser encontrado como solução otologica indicada no tratamento da otite externa parasitária e fungica em pequenos animais. Malassezia pachydermatis é uma levedura considerada habitante normalda pele e das mucosas de cães e gatos. Entretanto, considerase um agente do oportunista causador de otite externa edermatite nestes animais. A finalidade deste estudo foi avaliar o efeito in vitro do tiabendazol frente a 51 amostras de M. pachydermatis através do método CLSI de Microdiluição em Caldo adaptado para esta espécie de levedura (NCCLS, 2002). Baseado neste teste calculou-se as Concentrações InibitóriasMínimas (CIM) do tiabendazol e, subseqüentemente, foi detectada a suscetibilidade de cada amostra frente a este antifungico. Observou-se que a CIM do tiabendazol frente a M. pachydermatis variou de 0,03 a > 4 μg/mL. Estas amostras foram classificadas em resistente (13,7%), sensibilidade intermediária (47,1%) e sensível (39,2%). A resistência dalevedura frente ao tiabendazol mostrou similaridade com resultados anteriormente observados com outros antifúngicos e a adaptação da técnica de Microdiluição em Caldo utilizada mostrou-se eficiente.

Fotodegradación de las aguas residuales con pesticida Mertect en la industria bananera empleando Fotocatálisis con Dióxido de Titanio y Lámpara de Luz Ultravioleta / Degradation of waste waters from the banana industry by the use of photocatalysis with titanium dioxide and ultra violet light lamp

Introducción. Los pesticidas son compuestos químicos sintéticos que poseen la característica de ser persistentes, lo cual favorece la contaminación de las aguas, la incorporación a suelos, pastizales, vegetales y animales comestibles, los que al ser consumidos actúan como transportadores de los pesticidas facilitando su acumulación en los organismos vivos en todos los eslabones de la cadena trófica; es por esta razón que reviste importancia los procesos de degradación y descontaminación de este tipo de contaminantes. Objetivo. Evaluar la fotodegradación del pesticida Mertect empleando fotocatálisis con dióxido de titanio y lámpara de luz ultravioleta. Materiales y métodos. En la experimentación se utilizó una lámpara de luz ultravioleta, una cuba de vidrio para el almacenamiento de la solución del pesticida a degradar y una bomba que permitía la recirculación de la solución por el sistema de tratamiento; la degradación del pesticida se determinó mediante espectrofotometría ultravioleta/visible. Para el análisis de la información se empleo el diseño experimental factorial 2³, completamente balanceado. Resultados. El porcentaje de remoción más alto fue de 99,5%; este se obtuvo para dos condiciones experimentales: 50 mg/L de dióxido de titanio y 1 %v/v de peróxido de hidrógeno, y 100 mg/L de dióxido de titanio y 1 %v/v de peróxido de hidrógeno. Conclusión. Los procesos de oxidación avanzados son adecuados para la remoción y eliminación del pesticida Mertect en las aguas residuales de la industria bananera.

Introduction. Pesticides are synthetic chemical compounds with the characteristic of being persistent, thus helping to contaminate water and incorporating pollutants to the soils and to edible vegetables and animals that, when eaten, act as pesticide transporters, accumulating that pesticide in living organisms from all the links of the trophic chain. This is the reason for the great importance of the degradation and decontamination processes of this kind of pollutants. Objective. To evaluate the photodegradation of the Mertect pesticide by the use of photocatalysis with titanium dioxide and ultra violet light. Materials and methods. In the experiment, an ultra violet light lamp, a glass bucket to store the pesticide to be degraded and a bomb to recirculate the solution trough the treatment system were used. The degradation of the pesticide was determined by visible/ultraviolet spectrophotometry. For the information analysis the 2³ experimental factorial designs was used, under complete balance. Results. The highest removal percentage was 99.5%, obtained for two experimental conditions: 50 mg /L of titanium dioxide and 1% v/v of hydrogen peroxide, and 100mg/L of titanium dioxide and 1%v/ v of hydrogen peroxide. Conclusions. Advanced oxidation processes are adequate to remove and eliminate the Mertect pesticide in the waste waters from the banana industry.

Genotoxicidad de tres plaguicidas utilizados en la actividad bananera de Costa Rica / Genotoxicity of three pesticides used in Costa Rican banana plantations

The in vitro genotoxicity of imazalil and thiabendazole fungicides and the insecticide chlorpyrifos, compounds used in Costa Rican banana plantations, was evaluated with the single-cell gel electrophoresis technique (comet assay). The comet assay is a simple, rapid and low cost technique for quantification of DNA damage. This assay detects DNA single-strand breaks and alkali-labile sites in individual cells. The effects were analyzed by using human lymphocytes exposed to doses of 0, 25, 50, 75 and 100 microg/ml of each pesticide for 30 min at 37 degrees C. The cells were embedded in agarose, lysed, subjected to alkaline electrophoresis (pH >13) for 20 min at 25V, neutralized and dehydrated to be stained with a fluorescent dye and later comets visualization with the epifluorescence microscope. Chlorpyrifos and imazalil induced significant DNA damage in a dose-dependent manner. Chlorpyrifos was the major inductor of DNA breaks. These results indicate that both are genotoxic compounds in vitro. Thiabendazole fungicide did not induced DNA damage using the comet assay for all concentrations tested.

In vitro study on thiabendazole action on viability of Ascaris lumbricoides (Lineu, 1758) eggs

The in vitro activity of thiabendazole on Ascaris lumbricoides eggs, which were recovered from uteri of worm excreted after chemotherapeutic treatment, was studied. Four concentrations of the drug were used: 1 - 2.5 - 5 - and 10 ppm during 24, 48 and 72 hours of exposure. Subsequently, the eggs were centrifuged, washed three times and H(2)SO(4)0.1N was added. The eggs were maintained in an incubator for 20 days at 28 degrees C. Finally, the percentage of embryonated eggs was determined under a lightmicroscope at a 100X magnification. After 48 and 72 hours of thiabendazole exposure, at a concentration of 10ppm, the drug showed complete inhibition of egg embryonation.

Foi estudada, in vitro, a ação do tiabendazol, contra ovos de Ascaris lumbricoides , retirados de úteros de vermes eliminados após tratamento. Foram utilizadas quatro concentrações da droga: 1 ¾ 2,5 - 5 e 10 ppm em três diferentes períodos de tempo: 24, 48 e 72 horas. Decorridos estes tempos a suspensão com os ovos foi centrifugada, lavada por três vezes com água destilada, adicionada de solução de H2SO4, 0,1N e mantida em estufa a 28°C por 20 dias. Em seguida, os ovos foram levados ao microscópio óptico, no aumento de 100X, para observação do percentual de embrionamento. Na concentração de 10ppm, nos tempos de 48 e 72 horas de exposição à droga, verificou-se completa inibição do embrionamento dos ovos.